The main aim of present study was to evaluate the antibacterial activity of methanolic extract of Azadirachta indica against Xanthomonas citri on different level of concentration. The plant was selected on the basis of their reported medicinal uses. Each part of A. indica contains various pharmacological active substances against many diseases. In India most of the plant pathogenic diseases were caused by bacteria. X. citri is a plant pathogenic bacteria, severely affects citrus crop worldwide. In our lab work some antibacterial effective results have observed by the use of methanolic extract of A. indica. The results of inhibitory activity of the methanolic extract of A. indica compare with the standard antibiotic Kanamycin. The zone of inhibition of extract of A. indica at the concentration of 200 µg/ml was 20 mm (leaves) and the zone of inhibition of the standard antibiotic Kanamycin was 32mm. Hence, the result of A. indica (leaves) indicated that it may be used for treating plant diseases as natural bactericides